"hoechst and pi"怎么统计凋亡细胞 - 百度知道
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实验方法 Hoechst-PI染色法检测细胞凋亡 亚逗G1 地峰方法简便，但只是代表G0 /G1 期发生凋亡的细胞，S期和G2 期细胞凋亡发生于G1 峰后，无法观察。此外，由于全部细胞均经固定，因此不能区别死细胞和活细胞。Hoechst-PI染色则可弥补上述不足。Hoechst 33258 可被活细胞摄取，与DNA结合在紫外光下呈蓝色 ...
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