抗体蛋白纯化中的缓冲剂选择及其影响因素-公司新闻-艾伟拓 ...
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在常见的蛋白A亲和层析条件下，高离子浓度和高温会导致抗体蛋白聚集突出增加；缓冲体系选择方面，与醋酸盐和甘氨酸缓冲液相比，柠檬酸缓冲液倾向于导致更高的蛋白聚集。 而在离子交换层析中，离子强度、温度及pH值对抗体蛋白的电荷异质性有较大影响。
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