正相色谱柱与反相色谱柱是如何界定的,各适合那类物质的分离? - 知乎
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c18柱是反相色谱柱,cn柱是正相色谱柱。 正相色谱是采用极性固定相(如带有二醇基、氨基、和氰基的固定相及硅胶、三氧化二铝等)、非极性流动相(如正己烷等)的分离方法。这是一种根据分子的极性大小将其分开的液相色谱技术。
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