蛋白质DNA互作研究,选ChIP还是CUT&Tag? - 知乎

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CUT&Tag技术的全称是Cleavage Under Targets and Tagmentation,是一种验证DNA-蛋白互作的全新技术,是CUT&RUN的2.0版本。CUT&Tag完全颠覆传统ChIP-seq(染色质免疫沉淀-测序)的操作流程,它能够高效、准确地检测细胞中的蛋白质与DNA之间的相互作用。 CUT&Tag CUT&Tag技术的原理是在抗体引导下,Tn5酶仅在目的组蛋白 ...


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