ELISA实验标准曲线不佳，怎么排查原因？ - 知乎
16 June 2025 admin
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如何判断我绘制的标准曲线是否有效呢？ 在显色阶段，控制标准品前4孔有明显的蓝色梯度，以及标准品最大OD值≥1.2，标准曲线的线性回归相关系数R2≥0.99，即可作为有效标曲。 标准曲线的制作，可参考往期内容《ELISA实验指南！！新手必读》 但是，在ELISA实验过程中，往往有 标曲异常 的情况出现 ...
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