ELISA实验设阴性对照，是否需空白对照？ - 知乎
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ELISA实验的原理 1.包被：抗原或者抗体能以物理性吸附于固相载体表面，原因是蛋白质和聚苯乙烯表面间的疏水性部分相互吸附，吸附之后能保持抗原反应等免疫活性： 2、标记：抗原或者抗体可通过共价键与酶连接成酶结合物而此种酶结合物仍能保持其免疫活性和酶的催化特点： 3、显色：酶结合物 ...
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