怎样在NCBI的primer blast里设计Q-PCR的引物-百度经验
17 July 2025 admin
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首先打开NCBI，选择“Nucleotide”，并在搜索框输入基因名。一般用目标基因的突变体1去设计引物，也就是要选择目标基因的“transcript variant 1, mRNA ”,没有1就用2、3，以此类推。因为是Q-PCR，所以要去掉内含子，因此用mRNA。
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