Western Blot 一抗孵育4℃过夜,有时间范围吗?时间长度的上限或下限-丁香实验

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WesternBlot一抗孵育4℃过夜,有时间范围吗?时间长度的上限或下限;;主要是要看个人实验,根据蛋白和抗体性质决定,过夜是大部分实验要求,一般不超过12小时(abcam和赛信通),当然也有例外,有个博后师兄做线粒体自噬,因为新冠孵了3


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