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Total Phenol Content (TPC) was determined according to the Folin-Ciocalteu colorimetric method with some modifications. 32,33 Reaction mixtures were prepared with 1 mL of stock solutions of each extract, 0.5 mL Folin-Ciocalteu reagent (previously diluted 10-fold with distilled water) and 2.5 mL 7.5% sodium carbonate.
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