免疫沉淀 (IP) 技术指南和实验方案 - Thermo Fisher Scientific
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当使用蛋白A、G 和A/G时,可以使用特定的结合缓冲液提升抗体结合能力。 尽管在大多数生理缓冲液中均可发生结合,但专用的蛋白A 和蛋白G结合缓冲液可以提高结合水平,在某些情况下可以使结合能力至少翻倍 ,不过这些缓冲液未必都适用于抗原或蛋白相互作用。
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