第三代测序技术（Nanopore Sequencing）简述 - 知乎
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上图展示了ONT公司推出的MinION测序仪的详细工作原理。 在测序之前，将接头小分子连接到基因组 DNA 或 cDNA 片段的两端。 这些接头有助于DNA链被纳米孔附近的锚点蛋白（图a中纳米孔左侧的小分子）捕获，并辅助在一条链的 5' 端加载过孔蛋白酶，即phi29 DNA polymerase等。 该酶可以在毫秒时间尺度内沿链进行单向单核苷酸位移，确保检测器可以检测到稳定、足够用于分辨核苷酸类别的电信号。 当DNA分子在过孔蛋白酶的帮助下通过纳米孔时，传感器检测到由占据孔的核苷酸序列的差异引起的电流信号的变化（如图b）。
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