HPLC-QTOF/MS联用技术的原 理、应用及代谢组学介绍 ...

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HPLC-QTOF/MS联用技术的原 理、应用及代谢组学介绍. 王文昭 中科院微生物研究所真菌学国重室 wangwz@im.ac.cn. 提纲. HPLC-QTOF/MS联用技术的原理及应用. 分析方法的建立与验证. 代谢组学的基本策略及重要应用. 液质联用仪平台对所内外服务网页. ES中干燥气设置的作用. 单电荷离子. 检测到的离子种类依赖于溶剂添加剂 和分析所用的条件. CID质谱图解析——常见的中性丢失. 常见的A+2同位素峰值. 一元多项式展开式. Cl=3:1,(3+1)2=9+6+1. Br=1:1,(1+1)1=1+1. [M+H]+离子的N规则. 分析方法的建立. 液相色谱分析在线系统流程示意图. 分析方法的验证.


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