如何使用一个质粒载体同时表达两个基因？ - 知乎
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pet-duet质粒图谱 可以将两个基因分别插入两个多克隆位点，载体上有自带的启动子和终止子，也有相应的RBS序列。 只用一个多克隆位点插入两个基因在很大程度上很难实现将两个蛋白都分别表达，在他们之间分别加入RBS听起来理论上确实可行。但是做实验做起来你就知道有多难。基因上的一些结构 ...
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